Nucleic Acid Extractions
Our Nucleic Acid Extractions can be combined with sequencing, GWAS arrays, panels, or custom content products to provide you with an end-to-end solution for your study regardless of sample source. We offer extraction and qualification services for a wide range of sources and formats, including FFPE tissue.
DNA Extraction Get a Quote
- High Quality DNA Extraction from a variety of source materials
- Flexible formats and input types
- DNA quality and quantity for downstream applications
Details
| Source Material | Recommended Input | Product Compatibiliity |
|---|---|---|
| Blood | 1 mL | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| Saliva | 4 mL Oragene OG-500 | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| Cell Pellets | 1-2 x 106 cells | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| Frozen Tissue | 20-25 mg | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| FFPE Tissue Sections | 6-8 sections 10 μm thick, 250 mm2 surface area | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| FFPE Tissue Scrolls | 2-3 scrolls 30 μm thick, 250 mm2 surface area | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| FFPE Tissue Cores | 4-6 cores, 2 mm diameter | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| Blood Spots | 7 punches from FTA card | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| Buffy Coats | 200 μL | WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
For input levels less than the recommended amounts, please contact us
RNA Extraction Get a Quote
- High Quality RNA Extraction from a variety of source materials
- Flexible formats and input types
- RNA quality and quantity for downstream applications
Details
| Source Material | Recommended Input | Product Compatibility |
|---|---|---|
| PAXgene Blood | 10 mL PAXgene tube | WTS |
| Cell Pellets | 1-2 x 106 cells | WTS |
| Frozen Tissue | 20-25 mg | WTS |
| FFPE Tissue Sections | 6-8 sections 10 μm thick, 250 mm2 surface area | Transcriptome Capture |
| FFPE Tissue Scrolls | 2-3 scrolls 30 μm thick, 250 mm2 surface area | Transcriptome Capture |
| FFPE Tissue Cores | 4-6 cores, 2 mm diameter* | Transcriptome Capture |
For input levels less than the recommended amounts, please contact us
DNA + RNA Extraction Get a Quote
- High Quality DNA and RNA Extraction from the same source material
- Useful for multi-dimensional analysis of a single sample source
- FFPE compatible
Details
| Source Material | Recommended Input | Product Compatibility |
|---|---|---|
| Frozen Tissue | 20-25 mg | WTS, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| FFPE Tissue Sections | 6-8 sections 10 μm thick, 250 mm2 surface area | Transcriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| FFPE Tissue Scrolls | 2-3 scrolls 30 μm thick, 250 mm2 surface area | Transcriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| FFPE Tissue Cores | 4-6 cores, 2 mm diameter | Transcriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
| Cell Pellets | 1-2 x 106 cells | WTS, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels |
For input levels less than the recommended amounts, please contact us
Liquid Biopsy Extraction and QC Get a Quote
- Designed to compliment Liquid Biopsy Sequencing
- 5-10ml blood, 4-6ml plasma, 5-50ng cfDNA
- Fractionation of Germline and Somatic material
Details
Designed to compliment our Liquid Biopsy Exome and Panel Sequencing services, we use state-of-the-art optics based automation platforms to reliably fractionate and extract blood and plasma samples for liquid biopsy sequencing. As part of this extraction, we prepare a WGS library from the somatic sample which is then sequenced to ultra-low levels, allowing determination of the allele frequency (AF). We deliver this sequence data to you as part of the full liquid biopsy service. You can think of this as an incredibly data rich QC. Samples with under 10% AF are recommended for Gene Panel Sequencing. Samples over 10% AF are recommended for cfWES.
Stool Extraction (DNA + RNA) Get a Quote
- High Quality DNA/RNA Extraction from stool
- Can accommodate fresh or frozen stool
- Can accommodate large or small aliquots
- Preferred input is pure stool sample
Details
Using the QIAGEN AllPrep PowerFecal DNA/RNA Kit, we can purify both genomic DNA and total RNA from one sample source.
| EXTRACTION TYPE | Recommended Input | Product Compatibility |
|---|---|---|
| Without dissection / chipping | 75-100mg fresh or frozen stool | Microbial WGS |
| With dissection / chipping | >100mg fresh or frozen stool | Microbial WGS |
Exome Sequences Completed in 2015
Additional materials
Product specifications
