Nucleic Acid Extractions

Our Nucleic Acid Extractions can be combined with sequencing, GWAS arrays, panels, or custom content products to provide you with an end-to-end solution for your study regardless of sample source. We offer extraction and qualification services for a wide range of sources and formats, including FFPE tissue.

DNA Extraction Get a Quote

  • High Quality DNA Extraction from a variety of source materials
  • Flexible formats and input types
  • DNA quality and quantity for downstream applications

Details

Source MaterialRecommended InputProduct Compatibiliity
Blood1 mLWES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Saliva4 mL Oragene OG-500WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Cell Pellets1-2 x 106 cellsWES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Frozen Tissue20-25 mgWES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Sections6-8 sections 10 μm thick, 250 mm2 surface areaWES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Scrolls2-3 scrolls 30 μm thick, 250 mm2 surface areaWES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Cores4-6 cores, 2 mm diameterWES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Blood Spots7 punches from FTA cardWES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Buffy Coats200 μLWES, WGS, GWAS Arrays, Cancer Panel, Custom Panels

For input levels less than the recommended amounts, please contact us

RNA Extraction Get a Quote

  • High Quality RNA Extraction from a variety of source materials
  • Flexible formats and input types
  • RNA quality and quantity for downstream applications

Details

Source MaterialRecommended InputProduct Compatibility
PAXgene Blood10 mL PAXgene tubeWTS
Cell Pellets1-2 x 106 cellsWTS
Frozen Tissue20-25 mgWTS
FFPE Tissue Sections6-8 sections 10 μm thick, 250 mm2 surface areaTranscriptome Capture
FFPE Tissue Scrolls2-3 scrolls 30 μm thick, 250 mm2 surface areaTranscriptome Capture
FFPE Tissue Cores4-6 cores, 2 mm diameter*Transcriptome Capture

For input levels less than the recommended amounts, please contact us

DNA + RNA Extraction Get a Quote

  • High Quality DNA and RNA Extraction from the same source material
  • Useful for multi-dimensional analysis of a single sample source
  • FFPE compatible

Details

Source MaterialRecommended InputProduct Compatibility
Frozen Tissue20-25 mgWTS, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Sections6-8 sections 10 μm thick, 250 mm2 surface areaTranscriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Scrolls2-3 scrolls 30 μm thick, 250 mm2 surface areaTranscriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Cores4-6 cores, 2 mm diameterTranscriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Cell Pellets1-2 x 106 cellsWTS, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels

For input levels less than the recommended amounts, please contact us

Liquid Biopsy Extraction and QC Get a Quote

  • Designed to compliment Liquid Biopsy Sequencing 
  • 5-10ml blood, 4-6ml plasma, 2-20ng cfDNA

  • Fractionation of Germline and Somatic material

 

 

Details

Designed to compliment our Liquid Biopsy Exome and Panel Sequencing services, we use state-of-the-art optics based automation platforms to reliably fractionate and extract blood and plasma samples for liquid biopsy sequencing. As part of this extraction, we prepare a WGS library from the somatic sample which is then sequenced to ultra-low levels, allowing determination of the allele frequency (AF). We deliver this sequence data to you as part of the full liquid biopsy service. You can think of this as an incredibly data rich QC. Samples with under 10% AF are recommended for Gene Panel Sequencing. Samples over 10% AF are recommended for cfWES. 

Exome Sequences Completed in 2015

2,116

Additional materials

Nucleic Acid Extractions Product Specifications (117kb/pdf)

Product specifications