Walk-Up Sequencing

Walk-Up Sequencing is a quick and easy sequence data generation and delivery service for pre-constructed DNA libraries. We offer a variety of sequencing platforms and flowcell configurations to meet the needs of any project.  

If you are a Broad user, you can find the submission page here.

HiSeq 2500 High Yield Get a Quote

  • HiSeq 2500 up to 50 Cycles*
  • HiSeq 2500 up to 100 Cycles*
  • HiSeq 2500 up to 152 Cycles*
  • HiSeq 2500 up to 202 Cycles*

*Total cycle count, not including index read(s).

Details

Utilizing HiSeq SBS v4 chemistry, the HiSeq2500 High Yield service typically produces between 1.7 and 2 billion single reads or 3.4 to 4 billion paired-end reads per flowcell. Ideal applications include whole genome and exome sequencing.

HiSeq 2500 Rapid Get a Quote

  • HiSeq 2500 up to 50 Cycles*
  • HiSeq 2500 up to 200 Cycles*
  • HiSeq 2500 up to 250 Cycles*
  • HiSeq 2500 up to 300 Cycles*
  • HiSeq 2500 up to 500 Cycles* (250 cycles paired end)

*Total cycle count, not including index read(s).

Details

Utilizing HiSeq SBS v2 chemistry, the HiSeq2500 Rapid service typically produces between 270 and 300 million reads or 540-600 million paired-end reads per flowcell. Run time ranges between 7 and 60 hours depending on the number of cycles selected.

MiSeq Get a Quote

  • MiSeq up to 50 Cycles*
  • MiSeq up to 300 Cycles*
  • MiSeq up to 500 Cycles*
  • MiSeq up to 600 Cycles*

*Total cycle count, not including index read(s).

Details

Utilizing the MiSeq v2 reagent sets, the MiSeq service typically produces between 12 and 15 million reads or 24-30 million paired-end reads per sequencing run. Recommended applications include; targeted gene (amplicon, gene panel), small whole genome sequencing (microbe, virus), targeted RNA, small RNA, and 16s.

NextSeq 500 Get a Quote

  • NextSeq 500 up to 75 Cycles*
  • NextSeq 500 up to 150 Cycles*
  • NextSeq 500 up to 300 Cycles*

*Total cycle count, not including index read(s).

Details

Utilizing the NextSeq 550 High-Output reagent kits, the NextSeq service typically produces between 350 and 400 million single reads or 700-800 million paired-end reads per flowcell. The NextSeq is vertisile across a wide range of applications including; low scale whole genome, exome, targeted RNA and transcriptome sequencing, ChIP and Methyl Seq.

Example outputs (using 300 cycle kit): 1 human whole genome, 12 exomes, 16 transcriptomes

NovaSeq 6000 Get a Quote

  • NovaSeq SP up to 100 Cycles*
  • NovaSeq SP up to 300 Cycles*
  • NovaSeq SP up to 500 Cycles*
  • NovaSeq S1 up to 100 Cycles*
  • NovaSeq S1 up to 200 Cycles*
  • NovaSeq S1 up to 300 Cycles*
  • NovaSeq S2 up to 100 Cycles*
  • NovaSeq S2 up to 200 Cycles*
  • NovaSeq S2 up to 300 Cycles*
  • NovaSeq S4 up to 300 Cycles*

*Total cycle count, not including index read(s).

Details

Offering every flowcell type available and wide variety read lengths you will have no problems leveraging the most advanced sequencing platform commercially available. 

Flow Cell TypeSPS1S2S4
Single-end Reads650-800 M1.3-1.6 B3.3-4.1B8-10 B
Paired-end Reads1.3-1.6 B2.6-3.2 B6.6-8.2B16-20 B

The NovaSeq is ideal for whole genome, whole exome, and transcriptome sequencing projects.

Exome Sequences Completed in 2015

2,116

Additional materials

Walk-Up Sequencing Datasheet (76kb/pdf)

Product datasheet with detailed information on using Walk-Up Sequencing as a service