Our Microbial Whole Genome Sequencing products leverage the power of multiplexing and the latest in sequencing technology to enable large-scale sequencing of microbial isolates and metagenomes. Optimized for high-throughput, with the ability to identify low frequency variants, genome rearrangements, and gene-level variation among isolates and metagenomes, our microbial whole genome sequencing products come in several coverage models.
- Appropriate for bacterial isolates
- ~0.25Gb of data per sample, approximately ~1 million reads per sample
- 2 x 150bp reads
- De-multiplexed, aggregated, unaligned BAM file
Used for low-coverage sequencing of bacterial isolates, our standard MGX WGS 0.25Gb product provides approximately ~1 million reads per sample. Sample preparation is achieved using a tagmentation method and includes 384 unique dual indexes, enabling us to multiplex efficiently with low input starting material. Tagmentation can be affected by GC content, and though there is no significant effect on profiling metagenomic communities, additional coverage may be needed for genome assembly of isolates with less than ~25% GC content. Samples are accepted into this workflow on risk.
- Appropriate for bacterial isolates and small/simple population metagenomics
- ~1.5Gb of data per sample, approximately ~6 million reads per sample
- 2 x 150bp reads
- Available expedited
- De-multiplexed, aggregated, unaligned BAM file
Used for medium-coverage sequencing of bacterial isolates or small, simple population metagenomic studies, our standard MGX WGS 1.5Gb product provides approximately ~6 million reads per sample. Sample preparation is achieved using a tagmentation method and includes 384 unique dual indexes, enabling us to multiplex efficiently with low input starting material. Tagmentation can be affected by GC content, and though there is no significant effect on profiling metagenomic communities, additional coverage may be needed for genome assembly of isolates with less than ~25% GC content. Samples are accepted into this workflow on risk.
- Appropriate for bacterial isolates and large/complex population metagenomics
- ~3Gb of data per sample, approximately ~12 million reads per sample
- 2 x 150bp reads
- Available expedited
- De-multiplexed, aggregated, unaligned BAM file
Used for high-coverage sequencing of large genome bacterial isolates and complex bacterial communities or metagenomic studies, our standard MGX WGS 3Gb product provides approximately ~12 million reads per sample. Sample preparation is achieved using a tagmentation method and includes 384 unique dual indexes, enabling us to multiplex efficiently with low input starting material. Tagmentation can be affected by GC content, and though there is no significant effect on profiling metagenomic communities, additional coverage may be needed for genome assembly of isolates with less than ~25% GC content. Samples are accepted into this workflow on risk.
- Appropriate for deep coverage of bacterial isolates and very large/complex metagenomics
- >3Gb of data per sample, >12 million reads per sample
- 2 x 150bp reads
- De-multiplexed, aggregated, unaligned BAM file
Used for deep coverage sequencing of bacterial isolates and complex bacterial communities or metagenomic studies, we pair our standard MGX WGS 3Gb product with additional NovaSeq flowcells to obtain coverage >12 million reads per sample. Samples are accepted into this workflow on risk, pricing is dependent on utilizing full flowcells.
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The Broad Institute offers microbial whole genome sequencing enabling large-scale comparative studies of bacterial isolates and metagenomes, with varying levels of coverage.
Choice of Library Construction (LC) method is critical for the accurate genome assembly of isolate species and profiling of metagenomic communities. The Broad Institute’s Genomics Platform has implemented a modified tagmentation-based whole-genome sequencing process for microbial samples (Nextera XT DNA Library Kit, Illumina).