Nucleic Acid Extractions

Our Nucleic Acid Extractions can be combined with sequencing, GWAS arrays, panels, or custom content products to provide you with an end-to-end solution for your study regardless of sample source. We offer extraction and qualification services for a wide range of sources and formats, including FFPE tissue.

DNA Extraction Get a Quote
  • High Quality DNA Extraction from a variety of source materials
  • Flexible formats and input types
  • DNA quality and quantity for downstream applications
Details
Source Material Recommended Input Product Compatibiliity
Blood 1 mL WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Saliva 4 mL Oragene OG-500 WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Cell Pellets 1-2 x 106 cells WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Frozen Tissue 20-25 mg WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Sections 6-8 sections 10 μm thick, 250 mm2 surface area WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Scrolls 2-3 scrolls 30 μm thick, 250 mm2 surface area WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Cores 4-6 cores, 2 mm diameter WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Blood Spots 7 punches from FTA card WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Buffy Coats 200 μL WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels

For input levels less than the recommended amounts, please contact us

RNA Extraction Get a Quote
  • High Quality RNA Extraction from a variety of source materials
  • Flexible formats and input types
  • RNA quality and quantity for downstream applications
Details
Source Material Recommended Input Product Compatibility
PAXgene Blood 10 mL PAXgene tube WTS
Cell Pellets 1-2 x 106 cells WTS
Frozen Tissue 20-25 mg WTS
FFPE Tissue Sections 6-8 sections 10 μm thick, 250 mm2 surface area Transcriptome Capture
FFPE Tissue Scrolls 2-3 scrolls 30 μm thick, 250 mm2 surface area Transcriptome Capture
FFPE Tissue Cores 4-6 cores, 2 mm diameter* Transcriptome Capture

For input levels less than the recommended amounts, please contact us

DNA + RNA Extraction Get a Quote
  • High Quality DNA and RNA Extraction from the same source material
  • Useful for multi-dimensional analysis of a single sample source
  • FFPE compatible
Details
Source Material Recommended Input Product Compatibility
Frozen Tissue 20-25 mg WTS, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Sections 6-8 sections 10 μm thick, 250 mm2 surface area Transcriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Scrolls 2-3 scrolls 30 μm thick, 250 mm2 surface area Transcriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
FFPE Tissue Cores 4-6 cores, 2 mm diameter Transcriptome Capture, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels
Cell Pellets 1-2 x 106 cells WTS, WES, WGS, GWAS Arrays, Cancer Panel, Custom Panels

For input levels less than the recommended amounts, please contact us

Liquid Biopsy Extraction and QC Get a Quote
  • Designed to compliment Liquid Biopsy Sequencing
  • 5-10ml blood, 4-6ml plasma, 5-50ng cfDNA
  • Fractionation of Germline and Somatic material
Details

Designed to compliment our Liquid Biopsy Exome and Panel Sequencing services, we use state-of-the-art optics based automation platforms to reliably fractionate and extract blood and plasma samples for liquid biopsy sequencing. As part of this extraction, we prepare a WGS library from the somatic sample which is then sequenced to ultra-low levels, allowing determination of the allele frequency (AF). We deliver this sequence data to you as part of the full liquid biopsy service. You can think of this as an incredibly data rich QC. Samples with under 10% AF are recommended for Gene Panel Sequencing. Samples over 10% AF are recommended for cfWES. 

Stool Extraction (DNA + RNA) Get a Quote
  • High Quality DNA/RNA Extraction from stool
  • Can accommodate fresh or frozen stool
  • Can accommodate large or small aliquots
  • Preferred input is pure stool sample
Details

Using the QIAGEN AllPrep PowerFecal DNA/RNA Kit, we can purify both genomic DNA and total RNA from one sample source.

EXTRACTION TYPE Recommended Input Product Compatibility
Without dissection / chipping 75-100mg fresh or frozen stool Microbial WGS
With dissection / chipping >100mg fresh or frozen stool Microbial WGS
Additional Materials