Walk-Up Sequencing is a quick and easy sequence data generation and delivery service for pre-constructed DNA libraries. We offer a variety of sequencing platforms and flowcell configurations to meet the needs of any project.
If you are a Broad user, you can find the submission page here.
- MiSeq up to 50 Cycles*
- MiSeq up to 300 Cycles*
- MiSeq up to 500 Cycles*
- MiSeq up to 600 Cycles*
*Total cycle count, not including index read(s).
Utilizing the MiSeq v2 reagent sets, the MiSeq service typically produces between 12 and 15 million reads or 24-30 million paired-end reads per sequencing run. Recommended applications include; targeted gene (amplicon, gene panel), small whole genome sequencing (microbe, virus), targeted RNA, small RNA, and 16s.
- NextSeq 500 up to 75 Cycles*
- NextSeq 500 up to 150 Cycles*
- NextSeq 500 up to 300 Cycles*
*Total cycle count, not including index read(s).
Utilizing the NextSeq 550 High-Output reagent kits, the NextSeq service typically produces between 350 and 400 million single reads or 700-800 million paired-end reads per flowcell. The NextSeq is vertisile across a wide range of applications including; low scale whole genome, exome, targeted RNA and transcriptome sequencing, ChIP and Methyl Seq.
Example outputs (using 300 cycle kit): 1 human whole genome, 12 exomes, 16 transcriptomes
- NovaSeq SP up to 100 Cycles*
- NovaSeq SP up to 300 Cycles*
- NovaSeq SP up to 500 Cycles*
- NovaSeq S1 up to 100 Cycles*
- NovaSeq S1 up to 200 Cycles*
- NovaSeq S1 up to 300 Cycles*
- NovaSeq S2 up to 100 Cycles*
- NovaSeq S2 up to 200 Cycles*
- NovaSeq S2 up to 300 Cycles*
- NovaSeq S4 up to 300 Cycles*
*Total cycle count, not including index read(s).
Offering every flowcell type available and wide variety read lengths you will have no problems leveraging the most advanced sequencing platform commercially available.
| Flow Cell Type | SP | S1 | S2 | S4 |
| Single-end Reads | 650-800 M | 1.3-1.6 B | 3.3-4.1B | 8-10 B |
| Paired-end Reads | 1.3-1.6 B | 2.6-3.2 B | 6.6-8.2B | 16-20 B |
The NovaSeq is ideal for whole genome, whole exome, and transcriptome sequencing projects.
(76kb/pdf)
Product datasheet with detailed information on using Walk-Up Sequencing as a service